Fig. 6

Effects of red light on the signal molecules in Shiraia sp. S9. A The relative fluorescence intensity of Ca²⁺ in S9. B Changes in the fluorescence of Ca²⁺. bright-field image (above) and fluorescence microscopy (below) of Fluo-3 AM-stained mycelia (400 ×). EGTA (5 mM) and LaCl₃ (2 mM) were added 30 min prior to the red light, respectively. The intensity of red light (627 nm) was 200 lx. The red light-induced eATP release (C) and H₂O₂ content (D) in the mycelium of S9. E The effect of eATP signal on NO content. PPADS (10 μM), RB (10 μM) were added 30 min prior to the red light treatment, respectively. F The effect of Ca²⁺ signal on NO content. Values are mean ± SD from three independent experiments (*p < 0.05 and **p < 0.01 vs. control. ^#p < 0.05 and ^##p < 0.01 vs. red light treatment, ten flasks per replicate)