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Engineering monooxygenase for efficient production of indirubin, a colorful drug

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Indirubin is a bis-indole compound for the treatment of chronic myelocytic leukemia. Drs. Liu and Wang presented a structure-guided method to improve the activity of a flavin-containing monooxygenase (bFMO) for efficient production of indirubin in Escherichia coli. A flexible loop interlocked with the active pocket through a helix and the substrate tunnel in bFMO were identified to be two reconfigurable structures to improve its activity. A combined modification at the two regions (K223R/D317S) achieved a 6.6-fold improvement in catalytic efficiency (kcat/Km) due to the enhanced π–π stacking interactions. Furthermore, an engineered E. coli strain was constructed by metabolic engineering, which could produce 860.7 mg/L (18 mg/L/h) indirubin, the highest yield ever reported.

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C1 Biotechnology

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 Biomanufacturing Driven by Synthetic Biology

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Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes.

BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including:

  • Biochemical and microbiological engineering
  • Biocatalysis and biotransformation
  • Biosynthesis and metabolic engineering
  • Bioprocess and biosystems engineering
  • Bioenergy and biorefinery
  • Cell culture and biomedical engineering
  • Food, agricultural and marine biotechnology
  • Bioseparation and biopurification engineering
  • Bioremediation and environmental biotechnology

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