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P450 monooxygenases for directed steroid hydroxylation


Steroid hydroxylation is one of the most important functionalized reactions because their derivatives enable a better biological activity compared to their less polar non-hydroxylated analogs. Prof. Ai-Tao Li and his co-workers at Hubei University (China) made a thorough summary of the bacterial cytochrome P450s for steroid hydroxylation via high expression in workhorses like Escherichia coli. The recent developments on engineering of bacterial cytochrome P450s for steroid hydroxylation was highlighted, in order to overcome the common problems encountered frequently in their industrial applications, such as wrong or insufficient regio- and/or stereoselectivity, low activity, narrow substrate range as well as insufficient thermostability.

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Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes.

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