Protective effects of Aureobasidium pullulans lysate on UV-damaged human skin fibroblasts and HaCaT cells
Ultraviolet (UV) rays can cause skin photoaging. In order to explore the protective effect and application potential of A. pullulans lysate on UV-damaged human skin fibroblasts and HaCaT cells, this study investigates the anti-aging and anti-inflammatory effects of A. pullulans lysate as well as the mechanism of anti-oxidative stress at the cellular and molecular levels, where the experimental results show that the A. pullulans lysate can effectively reduce the loss of extracellular matrix components, such as collagen and hyaluronic acid. It is also capable of scavenging excess ROS, thereby increasing the activity of catalase, decreasing the overexpression of intracellular matrix metalloproteinases, while decreasing the level of inflammatory factors, and reducing UV-induced apoptosis of HaCaT cells. Meanwhile, oxidative stress homeostasis is also regulated through the Nrf2/Keap1 and MAPK signaling pathways.