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Improving specific activity and pH stability of a xylanase by directed evolution

Xylanases have been used in food, paper, and pulp industries, and considered to be a key player in the biodegradation of xylan to valuable end products. However, most of the natural xylanases show poor activity at higher temperature and at higher pH (alkali). Therefore, it is strongly desired to modify the enzymes to meet such conditions for the increasing demands in industry.

Prof. Zhang and her group at Hubei University (Wuhan, China) successfully improved the specific activity and pH stability of the xylanase (XynHBN188A) originated from Bacillus pumilus HBP8 by direct evolution, where the enlarged catalytic channel of the mutant is beneficial for the substrates access and products release. The mutant XynHBN188A217 will be a potential candidate to be used for industrial application.

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Beyond Natural Enzymes: The Power of Directed Evolution
Deadline for submissions: 30th September 2019

Refining Bioresources for Sustainable Future
Deadline for submissions: 31st January 2020

Biocatalysis for Fuels and Chemicals
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Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes.

BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including:

  • Biochemical and microbiological engineering
  • Biocatalysis and biotransformation
  • Biosynthesis and metabolic engineering
  • Bioprocess and biosystems engineering
  • Bioenergy and biorefinery
  • Cell culture and biomedical engineering
  • Food, agricultural and marine biotechnology
  • Bioseparation and biopurification engineering
  • Bioremediation and environmental biotechnology

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