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An Archaeon Argonaute Cleaves ssDNA Using Diverse Guides

Recently Yan Feng and her coworks in Shanghai Jiaotong University successfully cloned a thermotolerant argonaute protein from archaeon Methanocaldococcus fervens (MfAgo) and overexpressed in Escherichia coli BL21 (DE3). MfAgo shows efficient endonuclease activity for ssDNA cleavage. In contrast to most known pAgos, which recognize only one type of guide, MfAgo uses diverse guides, including 5′-P ssDNA, 5′-OH ssDNA, and 5′-P ssRNA, to specifically cleave targets. 

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Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes.

BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including:

  • Biochemical and microbiological engineering
  • Biocatalysis and biotransformation
  • Biosynthesis and metabolic engineering
  • Bioprocess and biosystems engineering
  • Bioenergy and biorefinery
  • Cell culture and biomedical engineering
  • Food, agricultural and marine biotechnology
  • Bioseparation and biopurification engineering
  • Bioremediation and environmental biotechnology

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