Table 3 Primers used in this work
Primers | Sequences of oligonucleotides (5′–3′) |
|---|---|
dxs (s1) | GGGAATTCCATATGAGTTTTGATATTGCCAA |
dxs (a2) | GCGAATTCTTATGCCAGCCAGGCCTTGATTTTG |
idi (s) | CGGAATTCGAAGGAGATATACATATGCAAACGGAACACGTCATTTTATTG |
idi (a) | GCGCTCGAGGCTCACAACCCCGGCAAATGTCGG |
dxsidiBamHI(s) | CGCGGATCCGATGAGTTTTGATATTGCCAA |
dxsidiKpnI (a) | CGGGGTACCGCTCACAACCCCGGCAAATGTCGG |
dxsidiBamH I 1(s) | CGCGGATCCATGAGTTTTGATATTGCCAA |
LSBamH I(s) | CGCGGATCCGATGGAACGTCGTAGCGGTAA |
LS(a) | CGTAGAATTCTTATGCAAATGGTTCAAACA |
GPPS(S) | CCGGAATTCGAAGGAGATATACATATGTTCGACTTCAACAAATAC |
GPPS(A) | ACGCGTCGACTCAGTTCTGACGGAATGCAAC |
GPPSBamH I(s) | CGCGGATCCGATGTTCGACTTCAACAAATAC |
GPPSBamH I 1(s) | CGCGGATCCATGTTCGACTTCAACAAATAC |
GPPS1(A) | CCGGAATTCTCAGTTCTGACGGAATGCAAC |
LS1(S) | CCGGAATTCGAAGGAGATATACATATGGAACGTCGTAGCGGTAA |
LS1 (a) | CCGGAATTCTTATGCAAATGGTTCAAACA |
S81F-rev | AATCATCATGAATTAAAAAGTAAGCGTGGATACAC |
S81F-for | GTGTATCCACGCTTACTTTTTAATTCATGATGATT |
EcFPPSBamHIs | CGCGGATCCGATGGACTTTCCGCAGCAACT |
EcFPPSEcoRIa | CCGGAATTCTTATTTATTACGCTGGATG |
LSEcoRISDs | CGTAGAATTCAGAAGGAGATATACATATGGAACGTCGTAGCGGTAA |
EcFPPSEcoRIs | CGTAGAATTCAGAAGGAGATATACATATGGACTTTCCGCAGCAACT |
EcFPPSalI | ACGCGTCGACTTATTTATTACGCTGGATG |