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Bioresources and Bioprocessing

Table 2 The components of ‘3 + 1’ washing buffers

From: Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies

Washing buffer

Urea (M)

EDTA (mM)

Tris (mM)

NaCl (mM)

Triton X-100 (%)

pH of the buffer

Buffer I

2

10

20

100

1

8.5

Buffer II

4

10

20

0

2

8.5

Buffer III

2

10

20

0

1

9.5

Deionized water

0

0

0

0

0

6.5 to 7.0

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