Engineering of Corynebacterium glutamicum for growth and production of L-ornithine, L-lysine, and lycopene from hexuronic acids

Hadiati, Atika; Krahn, Irene; Lindner, Steffen N; Wendisch, Volker F

doi:10.1186/s40643-014-0025-5

Bioresources and Bioprocessing

Table 2 Gene expression analysis of C. glutamicum WT in CGXII minimal medium with 50 mM glucose ^a

From: Engineering of Corynebacterium glutamicum for growth and production of L-ornithine, L-lysine, and lycopene from hexuronic acids

	Gene ID	Description	M
Differentially expressed genes in the presence of D-glucuronate	cg3219	ldh, L-lactate dehydrogenase	1.7
	cg3303	Transcriptional regulator PadR family	1.6
	cg0580	Hypothetical protein	1.5
	cg2789	nrdH, glutaredoxin-like protein NrdH	−1.5
	cg2182	ABC-type peptide transport system, permease component	−1.5
	cg3300	Cation transport ATPase	−1.5
	cg2477	Hypothetical protein	−1.6
	cg1809	DNA-directed RNA polymerase subunit omega	−1.7
	cg0935	Hypothetical protein	−1.8
	cg1286	Hypothetical protein	−1.8
Differentially expressed genes in the presence of D-galacturonate	cg2313	idhA3, myo-inositol 2-dehydrogenase	2.0
	cg1118	Pyrimidine reductase, riboflavin biosynthesis	1.9
	cg0687	gcp, putative O-sialoglycoprotein endopeptidase	1.9
	cg1116	tdcB, threonine dehydratase	1.9
	cg1784	ocd, putative ornithine cyclodeaminase	1.9
	cg3096	ald, aldehyde dehydrogenase	1.9
	cg0792	Thioredoxin domain-containing protein	1.8
	cg0682	ATPase or kinase	1.7
	cg1003	fthC, 5-formyltetrahydrofolate cycloligase	1.7
	cg1134	pabAB, para-aminobenzoate synthase components I and II	1.7
	cg1438	ABC-type transport system, ATPase component (C-terminal fragment)	1.7
	cg2430	Hypothetical protein	1.7
	cg1560	uvrA, excinuclease ATPase subunit	1.7
	cg1014	pmt, protein O-mannosyltransferase	1.6
	cg1668	Putative membrane protein	1.6
	cg2625	pcaF, β-ketoadipyl CoA thiolase	1.6
	cg2094	Hypothetical protein	1.6
	cg1241	Hypothetical protein	1.5
	cg1876	Glycosyltransferase	1.5
	cg2417	Short-chain-type oxidoreductase	1.5
	cg3118	cysl, ferredoxin-sulfite reductase	−1.5
	cg0504	qsuD, shikimate 5-dehydrogenase	−1.5
	cg1740	Putative nucleoside-diphosphate-sugar epimerase	−1.5
	cg3225	Putative serine/threonine-specific protein phosphatase	−1.5
	cg2945	ispD, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	−1.5
	cg1614	Hypothetical protein	−1.5
	cg3427	parA1, ATPase involved in chromosome partitioning	−1.5
	cg1252	fdxC, ferredoxin	−1.5
	cg0518	hemL, glutamate-1-semialdehyde 2,1-aminomutase	−1.5
	cg2587	Phosphoglycerate dehydrogenase or related dehydrogenase	−1.6
	cg1551	uspA1, universal stress protein UspA	−1.6
	cg0059	pknA, serine/threonine protein kinase	−1.7
	cg0156	cysR, transcriptional activator of assimilatory sulfate reduction	−1.7
	cg0966	thyA, thymidylate synthase	−1.7
	cg0060	pbpA, D-alanyl-D-alanine carboxypeptidase	−1.7
	cg1045	Hypothetical protein	−1.7
	cg3119	fpr2, probable sulfite reductase (flavoprotein)	−1.8
	cg1253	Succinyldiaminopimelate aminotransferase	−1.8
	cg0045	Putative ABC-type transporter membrane protein	−1.8
	cg3117	cysX, ferredoxin-like protein	−1.8
	cg3430	Hypothetical protein	−1.9
	cg3115	cysD, sulfate adenylyltransferase subunit 2	−1.9
	cg1037	rfp2, RPF2 precursor	−1.9

^aStatistical evaluation was carried out with the t-test, where p ≤ 0.05, log expression ratio M ≥ 1.5 or ≤ −1.5, and signal intensity A ≥ 10. Values are averages of three independent cultivations.

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