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Bioresources and Bioprocessing

Table 2 Relative enzymatic activities of PDH, ICDH, and CcO after substrate(s) feeding (26 h) in different batches

From: Improving the stability of glutamate fermentation by Corynebacterium glutamicum via supplementing sorbitol or glycerol

Key enzyme

Fermentation batches

Batch #1

Batch #2

Batch #3

Batch #4

Batch #5

Batch #6

PDH

0.84 ± 0.057

0.58 ± 0.043

0.72 ± 0.052

0.72 ± 0.036

0.76 ± 0.078

0.72 ± 0.061

ICDH

1.18 ± 0.051

0.72 ± 0.039

0.97 ± 0.061

0.92 ± 0.032

1.24 ± 0.076

1.27 ± 0.092

CcO

0.50 ± 0.063

0.00

1.50 ± 0.089

0.79 ± 0.043

0.75 ± 0.057

0.25 ± 0.04

  1. Batch #1, normal batch (control); batch #2, abnormal batch without sorbitol or glycerol; batch #3, sorbitol co-fed with glucose; batch #4, 15 g/L sorbitol added in the initial medium; batch #5, glycerol co-fed with glucose; batch #6, 10 g/L glycerol added in the initial medium.
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