Table 1 BBD design for optimization of inulin hydrolysis as per coded and decoded values of parameters
Run number | Enzyme concentration (U/g) | Substrate concentration (%) | Time (h) | Predicted ln sugar released (μg/ml) | Experimental ln sugar released (μg/ml) |
|---|---|---|---|---|---|
1 | 0 (5.5) | 0 (9.25) | 0 (12) | 11.1801 | 11.2912 |
2 | −1 (1) | 0 (9.25) | −1 (0.5) | 8.2346 | 8.6759 |
3 | 1 (10) | 0 (9.25) | -1 (0.5) | 9.1342 | 9.2789 |
4 | −1 (1) | 1 (17.5) | 0 (12) | 11.2268 | 11.0284 |
5 | −1 (1) | 0 (9.25) | 1 (23.5) | 10.4456 | 10.3009 |
6 | 1 (10) | 0 (9.25) | 1 (23.5) | 11.6786 | 11.2373 |
7 | −1 (1) | −1 (1) | 0 (12) | 6.895 | 6.7968 |
8 | 1 (10) | 1 (17.5) | 0 (12) | 11.9228 | 12.0209 |
9 | 0 (5.5) | −1 (1) | −1 (0.5) | 5.3199 | 4.9767 |
10 | 0 (5.5) | 0 (9.25) | 0 (12) | 11.1801 | 10.7729 |
11 | 0 (5.5) | 0 (9.25) | 0 (12) | 11.1801 | 11.4764 |
12 | 1 (10) | −1 (1) | 0 (12) | 8.3317 | 8.5301 |
13 | 0 (5.5) | 1 (17.5) | −1 (0.5) | 9.8109 | 9.568 |
14 | 0 (5.5) | −1 (1) | 1 (23.5) | 8.2272 | 8.4701 |
15 | 0 (5.5) | 1(17.5) | 1 (23.5) | 11.659 | 12.0021 |