Model-based optimization of Scheffersomyces stipitis and Saccharomyces cerevisiae co-culture for efficient lignocellulosic ethanol production

Bioresources and Bioprocessing

Table 3 Ethanol production performance by single-strain culture of S. cerevisiae and S. stipitis in comparison with co-culture of S. cerevisiae/S. stipitis under optimal cell ratio

Strains	[EtOH] (g/l)	R ^c_ETOH (g/l h)	Y ^d_ETOH (g/g)
Rice straw feedstock^a
S. cerevisiae	11.10 ± 0.71	0.23 ± 0.01	0.41 ± 0.03
S. stipitis	11.64 ± 0.12	0.24 ± 0.00	0.39 ± 0.00
S. cerevisiae/S. stipitis ^b	14.69 ± 0.27	0.31 ± 0.01	0.46 ± 0.00
Sugarcane bagasse feedstock^a
S. cerevisiae	41.81 ± 0.64	0.44 ± 0.01	0.33 ± 0.01
S. stipitis	42.05 ± 0.36	0.44 ± 0.00	0.33 ± 0.01
S. cerevisiae/S. stipitis ^b	46.68 ± 0.09	0.49 ± 0.00	0.38 ± 0.02

^aBased on batch SSF of rice straw at 6 % WIS for 48 h and sugarcane bagasse at 10 % WIS for 96 h
^bCo-culture of S. cerevisiae and S. stipitis was carried out under optimal cell ratio as predicted by the model (shown in Table 2) for each type of feedstock
^cRate of ethanol is defined as overall productivity which is total ethanol produced divided by fermentation time
^dEthanol yield is based on total sugar available in the feedstock