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Table 3 Kinetic parameters for hydrolysis of Z derivatives by TH2-CP

From: Characterization of a novel metallocarboxypeptidase from Streptomyces cinnamoneus TH-2

 

K m mM

V max mmol min−1 mg−1

V max/K m

Z-Gly-Phe

1.49 ± 0.13

1.19 ± 0.06

0.8

Z-Gly-Leu

1.10 ± 0.14

1.40 ± 0.06

1.28

Z-Gly-Gly-Leu

4.97 ± 0.34

4.82 ± 0.39

0.97

  1. K m and V max values were calculated from the Hanes–Woolf plots obtained using the Michaelis–Menten equation. Values are the mean ± SD of three independent experiments