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Table 2 Crystallization information

From: Sequence similarity network analysis, crystallization, and X-ray crystallographic analysis of the lactate metabolism regulator LldR from Pseudomonas aeruginosa

Method Vapour diffusion
Plate type 24-well hanging-drop plate (Hampton research)
Temperature (K) 287
Protein concentration (mg mL−1) 10
Buffer composition of protein solution 25 mM Tris–HCl, pH 8.0, 300 mM NaCl, and 2 mM dithiothreitol
Composition of reservoir solution 2 % w/v PEG 8000, 0.1 M Tris–HCl, pH 8.5
Volume and ratio of drop (µL) 1.0:1.0
Volume of reservoir (µL) 160