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Bioresources and Bioprocessing

Table 2 Best LEH variants of libraries A and B as catalysts in the hydrolytic desymmetrization of epoxide 1 with formation of (R,R)- or (S,S)-2

From: Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies

Library

Code

Mutations

Favored enantiomer

ee %

c %

A

WT

 

(R,R)

2

99

SZ133

I80L

(R,R)

56

81

SZ197

I80 V

(R,R)

72

93

SZ210

I80 V/V83L

(R,R)

77

96

SZ211

L114H/I116 V

(S,S)

80

95

SZ212

L114G/I116 V

(S,S)

74

77

SZ546

L114 V/I116 V

(S,S)

70

95

B

SZ717

L35 V

(S,S)

22

86

SZ213

L35 V/L74 V

(S,S)

37

28

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