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Table 2 Best LEH variants of libraries A and B as catalysts in the hydrolytic desymmetrization of epoxide 1 with formation of (R,R)- or (S,S)-2

From: Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies

Library Code Mutations Favored enantiomer ee % c %
A WT   (R,R) 2 99
SZ133 I80L (R,R) 56 81
SZ197 I80 V (R,R) 72 93
SZ210 I80 V/V83L (R,R) 77 96
SZ211 L114H/I116 V (S,S) 80 95
SZ212 L114G/I116 V (S,S) 74 77
SZ546 L114 V/I116 V (S,S) 70 95
B SZ717 L35 V (S,S) 22 86
SZ213 L35 V/L74 V (S,S) 37 28