Table 5 Results of testing the best LEH mutants evolved for epoxide 1 as catalysts in the hydrolytic desymmetrization of further meso-substrates
Code | 1 | 3 | 5 | 7 | Substrate concentration (mM) | ||||
|---|---|---|---|---|---|---|---|---|---|
ee % | c % | ee % | c % | ee % | c % | ee % | c % | ||
WT | 3 (S,S) | 87 | 7 (R,R) | 69 | 22 (S,S) | 99 | 93 (R,R) | 98 | 5 |
SZ501 | 97 (S,S) | 96 | 83 (S,S) | 30 | 89 (S,S) | 97 | 19 (R,R) | 93 | |
SZ502 | 97 (S,S) | 93 | 94 (S,S) | 34 | 97 (S,S) | 96 | 19 (R,R) | 93 | |
SZ503 | 79 (R,R) | 97 | 51 (R,R) | 48 | 53 (R,R) | 99 | 94 (R,R) | 99 | |
SZ504 | 96 (S,S) | 93 | 87 (S,S) | 31 | 94 (S,S) | 97 | 24 (R,R) | 95 | |
WT | 2 (S,S) | 99 | 13 (R,R) | 64 | 18 (S,S) | 99 | 92 (R,R) | 99 | 10 |
SZ501 | 97 (S,S) | 98 | 94 (S,S) | 18 | 97 (S,S) | 91 | 21 (R,R) | 62 | |
SZ502 | 98 (S,S) | 95 | 94 (S,S) | 39 | 97 (S,S) | 96 | 22 (R,R) | 85 | |
SZ503 | 80 (R,R) | 97 | 51 (R,R) | 64 | 54 (R,R) | 99 | 95 (R,R) | 99 | |
SZ504 | 95 (S,S) | 98 | 90 (S,S) | 26 | 94 (S,S) | 91 | 27 (R,R) | 55 | |