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Table 5 Results of testing the best LEH mutants evolved for epoxide 1 as catalysts in the hydrolytic desymmetrization of further meso-substrates

From: Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies

Code 1 3 5 7 Substrate concentration (mM)
ee % c % ee % c % ee % c % ee % c %
WT 3 (S,S) 87 7 (R,R) 69 22 (S,S) 99 93 (R,R) 98 5
SZ501 97 (S,S) 96 83 (S,S) 30 89 (S,S) 97 19 (R,R) 93
SZ502 97 (S,S) 93 94 (S,S) 34 97 (S,S) 96 19 (R,R) 93
SZ503 79 (R,R) 97 51 (R,R) 48 53 (R,R) 99 94 (R,R) 99
SZ504 96 (S,S) 93 87 (S,S) 31 94 (S,S) 97 24 (R,R) 95
WT 2 (S,S) 99 13 (R,R) 64 18 (S,S) 99 92 (R,R) 99 10
SZ501 97 (S,S) 98 94 (S,S) 18 97 (S,S) 91 21 (R,R) 62
SZ502 98 (S,S) 95 94 (S,S) 39 97 (S,S) 96 22 (R,R) 85
SZ503 80 (R,R) 97 51 (R,R) 64 54 (R,R) 99 95 (R,R) 99
SZ504 95 (S,S) 98 90 (S,S) 26 94 (S,S) 91 27 (R,R) 55