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Bioresources and Bioprocessing
Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: Protein expression analysis of a high-demeclocycline producing strain of Streptomyces aureofaciens and the roles of CtcH and CtcJ in demeclocycline biosynthesis

Fig. 4

Deletion of ctcH and ctcJ genes. a Schematic representation of the in-frame deletion of ctcH and ctcJ. A 410-bp fragment of ctcH and a 254-bp fragment of ctcJ were replaced with the 1145-bp neo gene by double crossover. b Confirmation of the ctcH deletion and ctcJ deletion mutants with PCR amplification. c 6-DCT production during the fermentation of the parental strain DT1 and the ctcH-deleted, ctcJ-deleted strains

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