Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

Bioresources and Bioprocessing

Table 4 The pool sizes of coenzymes in P. pastoris G1HL on Glc and on Glc/Glu

Metabolite	Glc (μmol/gDCW)		Glc/Glu (μmol/gDCW)		Glc + Glu/Glc	P value of T test
Metabolite	Value	SD	Value	SD	Glc + Glu/Glc	P value of T test
ADP	0.19	0.09	0.61	0.25	3.12	0.09
AMP^a	0.55	0.06	2.38	0.72	4.36	0.05
CAMP	0.31	0.27	0.35	0.27	1.10	0.89
CMP	0.26	0.02	13.61	8.12	52.97	0.10
GMP^a	0.19	0.07	0.67	0.18	3.48	0.03
UMP	0.34	0.15	0.54	0.06	1.61	0.13
NAD^+a	0.27	0.00	0.91	0.04	3.33	0.00
NADH^a	0.15	0.01	0.32	0.04	2.13	0.01
NADP^+a	0.88	0.02	1.00	0.01	1.13	0.00
NADPH^a	0.60	0.05	0.77	0.06	1.29	0.03

^aThe pool sizes of metabolites in G1HL on Glc/Glu were significantly up-regulated or down-regulated compared to those on Glc by two-tailed T test with P = 0.05