Fig. 2

Fermentation characteristics of strains in SECS hydrolysate with different sources of nitrogen and initial inoculum sizes. The experiments were performed in triplicate in SECS hydrolysates supplied by Novozymes (Table 2). Cells were cultured at 30 °C in a shake flask with a rubber stopper and agitation at 200 rpm. Strain XH7 (Fig. 1b; Table 1) a with 0.5 g DCW L⁻¹ initial biomass in YP (10 g L⁻¹ yeast extract and 20 g L⁻¹ peptone); b with 0.5 g DCW L⁻¹ initial biomass in 5 g L⁻¹ urea; c with 1.5 g DCW L⁻¹ initial biomass in 5 g L⁻¹ urea; and d strain XHR11 with 1.5 g L⁻¹ initial biomass in 5 g L⁻¹ urea. Symbols: filled square glucose; filled diamond xylose; filled triangle xylitol; filled circle ethanol; open circle glycerol; filled star acetic acid; dash biomass (DCW dry cell weight)