Table 1 Strains and plasmids used in this study
Strain/plasmid | Genotype/properties | Resource/reference |
|---|---|---|
Saccharomyces cerevisiae | ||
 BSIF | Diploid S. cerevisiae strain isolated from tropical fruit in Thailand | Laboratory preserved (Li et al. 2015) |
 BSN3 | BSIF derivative; XKS1p:: loxP-TEF1p, gre3:: TPI1p-RKI1-RKI1t-PGK1p-TAL1-TAL1t-FBA1p-TKL1-TKL1t-ADH1p-RPE1-RPE1t-loxP, pho13:: (TEF1p-Ru-xylA-PGK1t) 3 -loxP, three rounds of δ-integration with a fragment containing three tandem expression cassettes of Ru-xylA and the selectable marker loxP-KanMX4-loxP | The present work |
 XH7 | Single-colony isolate from adaptive evolution in xylose; based on BSN3 | The present work |
 XHR11 | Single-colony isolate from adaptive evolution in SECS liquor; based on XH7 | The present work |
 XH7-N360F | XH7 derivative; gre3::MGT05196 N360F | The present work |
 XHR11-N360F | XHR11 derivative; gre3::MGT05196 N360F | The present work |
 LF1 | Single-colony isolate from adaptive evolution in xylose; based on XHR11-N360F | The present work |
Plasmids | ||
 pUG6 | E.coli plasmid with segment LoxP-KanMX4-LoxP | Güldener et al. (1996) |
 pJX7 | pJFE3; TEF1p-Ru-xylA-PGK1t | Bao et al. (2013) |
 pXIP1 | pUC19-based yeast integration plasmid containing the PHO13-targeting recombinant arms PHO13-RA1 and PHO13-RA2, three tandem expression cassettes of Ru-xylA and the selectable marker loxP-KanMX4-loxP | The present work |
 pXIP2 | Similar to pXIP1; the recombinant arms PHO13-RA2 in pXIP1 were replaced with PHO13-RA3 in pXIP2 | The present work |
 pXIδ | pUC19-based yeast integration plasmid containing the δ-sequence-targeting recombinant arms, three tandem expression cassettes of Ru-xylA and the selectable marker loxP-KanMX4-loxP | The present work |
 pJPPP3 | pUC19-based yeast integration plasmid containing the GRE3-targeting recombinant arms RA1/2; an expression cassette for Sc-RKI1, Sc-TAL1, Sc-TKL1 and Sc-RPE1; and the selectable marker loxP-KanMX4-loxP | Peng et al. (2012) |
 pJPPP4 | The GRE3-targeting recombinant arms GRE3-RA2 in pJPPP3 were replaced with GRE3-RA3 in pJPPP4 | The present work |
 pUC-N360F | pUC19-based yeast integration plasmid containing GRE3-targeting recombinant arms, an overexpression cassette for MGT05196 N360F, the upstream activating sequence (UAS elements) UASCLB, and the selectable marker loxP-KanMX4-loxP | The present work |
 YEp-CH | YEp24 derivative; GAL1p-Cre-CYC1t, TEF1p-hygB-TEF1t | Laboratory preserved |