Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin

Bioresources and Bioprocessing

Table 5 Intracellular NADH, NAD⁺ and ratios of NADH/NAD⁺ in MG1 and its derivatives

Strains	MG1		MG12		MG13		MG14
Fermentation time (h)	18	36	18	36	18	36	18	36
Intracellular NADH (μmol/l OD₆₀₀)	0.32 ± 0.01	0.25 ± 0.01	0.41 ± 0.02	0.37 ± 0.02	0.36 ± 0.02	0.33 ± 0.01	0.45 ± 0.02	0.41 ± 0.02
Intracellular NAD⁺ (μmol/l OD₆₀₀)	0.35 ± 0.01	0.28 ± 0.01	0.31 ± 0.01	0.25 ± 0.01	0.33 ± 0.01	0.28 ± 0.01	0.28 ± 0.01	0.25 ± 0.01
Ratio of NADH/NAD⁺	0.91 ± 0.01	0.89 ± 0.01	1.32 ± 0.02	1.48 ± 0.02	1.09 ± 0.02	1.18 ± 0.01	1.61 ± 0.02	1.64 ± 0.02