Hydrolysis of carotenoid esters from Tagetes erecta by the action of lipases from Yarrowia lipolytica

Bioresources and Bioprocessing

Table 3 Experimental and fitted data for the experimental design 2⁷⁻⁴

Treatment	Design coordinate^d	Lipolytic activity^a	Biomass^a	μ ^c	Specific yield of lipases^b
Treatment	Design coordinate^d	U/L	X _max (g/L)	h⁻¹	Y _p/x (U/g)
3	−++−+−−	1153.6	3.556	0.080	510.56
1	−−++−−+	944.5	4.972	0.122	254.24
2	+++++++	1029.06	4.589	0.123	301.39
2	+++++++	1229.4	4.179	0.124	375.68
3	−++−+−−	1273.4	3.428	0.086	507.64
4	−+−+−+−	1111.7	4.391	0.100	391.71
1	−−++−−+	1023.3	4.909	0.124	220.09
5	−−−−+++	945.4	4.181	0.126	287.19
6	+−−++−−	1018.0	4.819	0.149	253.20
7	++−−−−+	981.9	4.598	0.118	310.47
4	−+−+−+−	1121.2	4.345	0.097	338.53
5	−−−−+++	936.0	4.026	0.129	276.97
8	0	947.9	5.106	0.109	233.71
6	+−−++−−	1030.0	4.256	0.129	324.98
7	++−−−−+	1050.0	4.509	0.128	262.01
8	0	1100.4	4.521	0.094	313.86
9	+−+−−+−	805.1	5.425	0.122	174.16
9	+−+−−+−	888.3	4.594	0.157	284.31

^aMeasures at 40 h of culturing
^bComputed through the linear regression of lipolytic activity (U/L) vs X (g/L)
^cComputed through the logistic model
^d+ and − represent high and low level, respectively, for factors ABCDEFG. 0 stands for a medium level which results from the average of the maximum and minimum value