Table 5 Examples of whole-cell biocatalytic reactions in DES
From: Recent progress on deep eutectic solvents in biocatalysis
Microbial | DES | Substrate | Product | Comments | References |
|---|---|---|---|---|---|
Baker’s yeast | ChCl/Gly (1:2) | Ethyl acetoacetate | Ethyl 3-hydroxybutyrate | Increasing the DES content switched the enantioselectivity | Maugeri and de Maria (2014) |
Acetobacter sp. CCTCC M209061 | ChCl/U (1:2) | 3-Chloropropiophenone | (S)-3-Chloro-1-phenylpropanol | Combination of ILs and DESs in the biphasic system effectively improved the substrate concentration | Xu et al. (2015) |
Recombinant E. coli overexpressing ADH | ChCl/Gly (1:2) | Ketones | Alcohols | The e.e. of many aromatic substrates increased by the addition of DES | Muller et al. (2015) |
Baker’s yeast | ChCl/Gly (1:2) | Aryl-containing ketones | Aryl-containing alcohols | (S)-oxidoreductases of baker’s yeast was possibly inhibited by DES | Vitale et al. (2016) |
Recombinant E. coli CCZU-T15 | ChCl/Gly (1:2) | 4-chloro-3-oxobutanoate | (S)-4-chloro-3-hydroxybutanoate | Addition of Tween-80 improved significantly substrate concentration from 2 to 3Â M | Dai et al. (2017) |
Lysinibacillus fusiformis CGMCC 1347 | ChOAc/U (1:1) ChOAc/EG (1:1) ChCl/Lac (4:1) ChCl/Raf (11:2) | Isoeugenol | Vanillin | NADESs were first used in whole-cell biocatalysis | Yang et al. (2017) |