Fig. 7
From: Photocontrolled reversible self-assembly of dodecamer nitrilase
The fluorescence intensities of fusion protein-coexpressing strains. a A + light represents the strains coexpressing mN159, mC160, and SspB under blue light illumination; B + dark represents the strains coexpressing BCNIT-mN159-AsLOV2-SsrA (BNMnAS), BCNIT-mC160-AsLOV2-SsrA (BNMcAS), and SspB in the dark; C + light represents the strains coexpressing BNMnAS, BNMcAS, and SspB under blue light illumination. b Comparison of the nitrilase activities of BCNIT, BNAS, and BNASS in E. coli. Error bars represent the standard error of three replicates. The determination of nitrilase activity was performed at 30 °C for 30 min with 10 µM FMN under 470-nm blue light illumination. Under the experimental conditions, the relative activity was expressed as a percentage of the maximum activity. Error bars represent the standard error of three replicates