Fig. 5

Effects of pH on the activity and stability of the recombinant AcCR. Reaction conditions: a 0.25 mM NADH, 2 mL 50 mM buffer (pH 4.5–9.5), and 5 mM 4′-chloroacetophenone were incubated at 35 °C for 5 min before adding 20 μL purified recombinant AcCR (about 0.008 mg of the purified enzyme), and then the changes of absorbance for 3 min at 35 °C were recorded; b 0.5 mM NAD⁺, 2 mL 50 mM buffer (pH 5.5–9.5), and 150 mM isopropanol were incubated at 45 °C for 5 min before adding 20 μL purified recombinant AcCR (about 0.008 mg of the purified enzyme), and then the changes of absorbance for 3 min at 45 °C were recorded; c 0.25 mM NADH, 2 mL 50 mM buffer (pH 4.5–9.5), and 5 mM 4′-chloroacetophenone were incubated for 5 min at 35 °C, then 20 μL purified recombinant AcCR was added (about 0.008 mg of the purified enzyme), and the changes of absorbance for 3 min were recorded