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Bioresources and Bioprocessing

Table 1 Screening of enoate reductases for citral reduction

From: Identification of a yeast old yellow enzyme for highly enantioselective reduction of citral isomers to (R)-citronellal

Enzyme

Sources of strains

Conversions (%)

ee (%)

OYE2p

Saccharomyces cerevisiae YJM1341

97.2

84.5 (R)

OYE3p

Saccharomyces cerevisiae YJM1133

95.8

41.3 (R)

Hypothetical protein

Vanderwaltozyma polyspora DSM 70294

46.4

67.8 (R)

HYE2

Ogataea polymorpha

97.6

25.5 (R)

MgER

Meyerozyma guilliermondii

93.5

11.7 (R)

  1. Reaction conditions: 5 mM (E/Z)-citral, 40 g/L resting cells and 0.2 mM NAD+ were mixed in 1 mL PBS (200 mM, pH 7.4). The reactions were incubated at 30 °C and 300 rpm for 10 h
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