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Table 1 Screening of enoate reductases for citral reduction

From: Identification of a yeast old yellow enzyme for highly enantioselective reduction of citral isomers to (R)-citronellal

Enzyme Sources of strains Conversions (%) ee (%)
OYE2p Saccharomyces cerevisiae YJM1341 97.2 84.5 (R)
OYE3p Saccharomyces cerevisiae YJM1133 95.8 41.3 (R)
Hypothetical protein Vanderwaltozyma polyspora DSM 70294 46.4 67.8 (R)
HYE2 Ogataea polymorpha 97.6 25.5 (R)
MgER Meyerozyma guilliermondii 93.5 11.7 (R)
  1. Reaction conditions: 5 mM (E/Z)-citral, 40 g/L resting cells and 0.2 mM NAD+ were mixed in 1 mL PBS (200 mM, pH 7.4). The reactions were incubated at 30 °C and 300 rpm for 10 h