Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Yang, Yongfu; Hu, Mimi; Tang, Ying; Geng, Binan; Qiu, Mengyue; He, Qiaoning; Chen, Shouwen; Wang, Xia; Yang, Shihui

doi:10.1186/s40643-018-0193-9

Bioresources and Bioprocessing

Table 1 Ethanol fermentation by Z. mobilis in lignocellulosic hydrolysates compared to pure sugars

From: Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Carbon source	Pretreatment method	Z. mobilis strain	Fermentation	Sugar (g/L)	Inhibitors (g/L)	Ethanol titer (g/L)	References
Glucose		ZM4	Batch fermentation in 2.5-L bioreactor	100.0		44.9	Zhao et al. (2014)
Xylose		TSH-01	2-L bioreactor	80.0		> 30.0	Feng et al. (2012)
Glucose and xylose		ZM4 (pZB5)	Batch fermentation in 2-L fermentor	Glucose: 65.0 Xylose: 65.0		62.0	Joachimsthal et al. (1999)
Glucose, xylose, and mannose		Z. mobilis [sucZE2::manA, pZA22-xtR]	200-mL bioreactor	Glucose: 20.0 Xylose: 20.0 Mannose: 20.0		26.5	Yanase et al. (2012)
Glucose, xylose, and arabinose		AX101	BioStat-Q fermentors	Glucose: 40.0 Xylose: 40.0 Arabinose: 20.0		42.0	Mohagheghi et al. (2002)
Corn stover	DA	8b	500-mL bioreactor	Glucose: 14.5 Xylose: 90.9 Arabinose: 11.1 Galactose: 7.0	Acetic acid: 5.9 Furfural: 5.2 5-HMF: 0.2	> 60.0	Schell et al. (2016)
Corn stover	DA with NH₄OH-treated	8b	250-mL flasks	Glucose: 31.4 Xylose: 85.7 Fructose: 4.2 Arabinose: 11.2 Galactose: 6.6	Acetic acid: 16.3 HMF: 3.7 Furfural: 2.3	> 48.0^a	Jennings and Schell (2011)
Corn stover	DA	8b	BioStat-Q fermenters	Glucose: 16.0 Xylose: 69.0	Acetic acid: 11.0	36.6^b	Mohagheghi et al. (2004)
Corn stover	AFEX followed autoclaving	2302	500-mL bioreactor	Glucose: 63.9 Xylose: 31.7	Furfural: 6.5 5-HMF: 0.1 Vanillin: 21.5 Syringaldehyde: 1.3	39.1	Serate et al. (2015)
Switchgrass	AFEX followed autoclaving	2302	500-mL bioreactor	Glucose: 59.1 Xylose: 31.2	Furfural: 5.2 5-HMF: 0.1 Vanillin: 11.6 Syringaldehyde: 1.0	38.3	Serate et al. (2015)
Corn stover	DASE	ZM401	Batch fermentation in 2.5-L bioreactor	Glucose: 80.00 Xylose: 33.00	Furfural: 0.49 HMF: 0.28 Acetic acid: 0.66	> 27.5	Zhao et al. (2014)
Corn stover	DDAP	ZM4	3-L fermentor	Glucose: 58.36	Furfura: 0.26 HMF: 0.29 Formic acid: 0.92	15.0	Dong et al. (2013)
Corn stover	DA	TSH-01	2-L bioreactor	Glucose: 5.8 Xylose: 18.2 Arabinose: 1.1	Acetic acid: 7.5 Formic acid: 1.2 Furfural: 0.6	> 30.0	Feng et al. (2012)
Coniferous trees	DA	Z. mobilis [sucZE2::manA, pZA22-xtR]	Continuous fermentation Laboratory-scale bioreactor	Glucose: 68.5 Xylose: 14.3 Mannose: 22.4		37.3	Yanase et al. (2012)
Wheat straw	DA	ZM4 (pZB5)	Batch fermentation in 100-mL flasks	Glucose: 23.3 Xylose: 19 Arabinose: 3.0	Acetate: 3.1 Formate: 0.6 Levulinic acid: 0.7 HMF: 0.08 Furfural: 0.67	13.8	Jeon et al. (2010)
Bagasse	DA	ZM4 (pZB5)	Batch fermentation in 100-mL flasks	Glucose: 26.9 Xylose: 18.2 Arabinose: 2.8	Acetate: 4.1 Formate: 0.7 Levulinic acid: 0.8 HMF: 0.1 Furfural: 0.74	17.7	Jeon et al. (2010)
Sorghum straw	DA	ZM4 (pZB5)	Batch fermentation in 100-mL flasks	Glucose: 22.0 Xylose: 16.9 Arabinose: 3.7	Acetate: 2.3 Formate: 1.2 Levulinic acid: 2.6 HMF: 0.6 Furfural: 0.5	10.6	Jeon et al. (2010)
Sugarcane tops	DA	ZM4 (pZB5)	Batch fermentation in 100-mL flasks	Glucose: 22.3 Xylose: 18.7 Arabinose: 3.1	Acetate: 2.1 Formate: 0.2 Levulinic acid: 0.2 HMF: 0.14 Furfural: 0.14	10.3	Jeon et al. (2010)
Arundo donax	DA	ZM4 (pZB5)	Batch fermentation in 100-mL flasks	Glucose: 17.7 Xylose: 23.3 Arabinose: 2.2	Acetate: 4.8 Formate: 0.5 Levulinic acid: 0.2 HMF: 0.04 Furfural: 0.74	12.3	Jeon et al. (2010)
Oil mallee	DA	ZM4 (pZB5)	Batch fermentation in 100-mL flasks	Glucose: 10.6 Xylose: 3.4 Arabinose: 5.0	Acetate: 0.7 Formate: 0.5 Levulinic acid: 0.7 HMF: 0.05 Furfural: 0.96	3.8	Jeon et al. (2010)
Pine	DA	ZM4 (pZB5)	Batch fermentation in 100-mL flasks	Glucose: 10.6 Xylose: 19.7 Arabinose: 1.5	Acetate: 1.8 Formate: 0.6 Levulinic acid: 0.4 HMF: 0.14 Furfural: 0.15	0.2	Jeon et al. (2010)
Eucalyptus	DA	ZM4 (pZB5)	Batch fermentation in 100-mL flasks	Glucose: 8.9 Xylose: 14.6 Arabinose: 0.3	Acetate: 3.4 Formate: 0.3 Levulinic acid: 0.2 Furfural: 0.2	2.3	Jeon et al. (2010)
Sugar cane bagasse	6% NaOH/15% peracetic acid	CP4 (pZB5)	SSCF in 250-mL flask			21.1	Teixeira et al. (2000)
Hybrid poplar wood	6% NaOH/15% peracetic acid	CP4 (pZB5)	SSCF in 250-mL flask			21.3	Teixeira et al. (2000)
Rice straw	Concentrated acid	CP4 (pZB5)	Batch fermentation in 125-mL flasks	Glucose: 76.0 Xylose: 33.8		44.3	Krishnan et al. (2000)
Corn fiber	DA	CP4 (pZB5)				22.6	Bothast et al. (1999)

DA dilute acid, DASE dilute acid steam explosion, STEX steaming or steam explosion, AFEX ammonia fiber explosion, DDAP dry dilute acid pretreatment, SSF simultaneous saccharification and fermentation, ASSF advanced solid-state fermentation technology, SSCF simultaneous saccharification and co-fermentation, 5-HMF 5-hydroxymethyl furfural
^{a, b}Ethanol titers in these two studies were calculated from the reported ethanol yield of 80%^a and 85%^b based on the maximum theoretical yield of total amount of glucose and xylose to ethanol

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