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Table 1 Purification of recombinant enzyme Egls using PEG 4000-phosphate-NaCl system

From: Purification and characterizations of a novel recombinant Bacillus velezensis endoglucanase by aqueous two-phase system

Steps Volume Total protein (μg/ml) Total CMCase (U/ml) PF CR ER (%)
Crude enzyme 40 22,330.44 1105.65 12.05 3.18 90.39
Top phase 140 2611.30 ND    
Bottom phase 44 3235.91 1223.25    
  1. ND not detected