Table 1 Purification of recombinant enzyme Egls using PEG 4000-phosphate-NaCl system
Steps | Volume | Total protein (μg/ml) | Total CMCase (U/ml) | PF | CR | ER (%) |
|---|---|---|---|---|---|---|
Crude enzyme | 40 | 22,330.44 | 1105.65 | 12.05 | 3.18 | 90.39 |
Top phase | 140 | 2611.30 | ND | |||
Bottom phase | 44 | 3235.91 | 1223.25 |