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Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Self-cloning CRISPR/Cpf1 facilitated genome editing in Saccharomyces cerevisiae

Fig. 3

scCRISPR/Cpf1 facilitated singleplex genomic integration of in vivo assembled DNA parts in S. cerevisiae. a scCRISPR/Cpf1 facilitated singleplex genomic integration of the MEL1 into the Gal1-7. b Efficiency of singleplex genomic integration assisted by scCRISPR/Cpf1. c Pictures of transformants obtained by co-transforming donor DNA of MEL1 with pSC-Pal and cr-Gal17. d PCR result of 1 white transformant (−) and 6 blue transformants, lines #1–6, using primer pair SQ1/SQ2. a, b in orange rectangle: 50 bp homologous connector sequences. PTEF1, promoter of the TEF1; TTPS1, terminator of the TPS1

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