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Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30

Fig. 3

Cellulase production and a comparison of transcript levels of cellulase-related genes between strains TXYR1VP and RUT C30. FPase (A), pNPCase (B), CMCase (C), and pNPGase (D) activities of RUT C30 and TXYR1VP after a switch from glycerol to 20 g/L Avicel or lactose. Enzymatic activities were measured at 2, 3, and 4 days after the transfer. Error bars show the respective standard deviations of three biological replicates; asterisks indicate significant differences (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; n.s. not significant) between the transformants and RUT C30, as assessed by Student’s t test. Expression ratios of cellulase-related genes on 20 g/L Avicel (E) or lactose (F) for 12 and 24 h after the switch from glycerol. Data on TXYR1VP transformants were normalized to the corresponding gene expression levels at the same time points in RUT C30. Values represent the mean of three biological replicates, and the error bars denote standard deviations. Gene expression ratios greater than twofold or less than 0.5-fold are marked with “a”

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