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Table 1 Effect of different sources of glycerol on succinate production of the two-stage flask culture using the engineered strain MLB/pTrc99a-pck

From: Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation

Glycerol used in aerobic stage Glycerol used in anaerobic stage* Initial cell concentration (g DCW/L) Consumed glycerol (mmol/L) Amount of product (mmol/L) Yield of succinate (mol/mol)
Succinate Acetate Ethanol
Pure Pure 5.19 158.7 ± 10.2a 147.6 ± 6.9 2.6 ± 0.3 ND 0.93 ± 0.01
Without simple purification 5.03 126.4 ± 8.3 115.3 ± 7.9 NDb ND 0.91 ± 0.01
With simple purification 5.10 147.5 ± 1.5 138.4 ± 1.9 0.4 ± 0.1 ND 0.93 ± 0.01
Without simple purification Without simple purification 4.98 106.8 ± 1.3a 99.3 ± 0.6 3.2 ± 0.4 ND 0.93 ± 0.01
With simple purification With simple purification 5.07 140.7 ± 7.5 129.4 ± 4.7 2.5 ± 0.2 ND 0.92 ± 0.01
  1. ND Not detected
  2. * The anaerobic stage lasted 72 h
  3. aData are means ± standard deviations with three replicates