Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation

Bioresources and Bioprocessing

Table 1 Effect of different sources of glycerol on succinate production of the two-stage flask culture using the engineered strain MLB/pTrc99a-pck

Glycerol used in aerobic stage	Glycerol used in anaerobic stage*	Initial cell concentration (g DCW/L)	Consumed glycerol (mmol/L)	Amount of product (mmol/L)			Yield of succinate (mol/mol)
Glycerol used in aerobic stage	Glycerol used in anaerobic stage*	Initial cell concentration (g DCW/L)	Consumed glycerol (mmol/L)	Succinate	Acetate	Ethanol	Yield of succinate (mol/mol)
Pure	Pure	5.19	158.7 ± 10.2^a	147.6 ± 6.9	2.6 ± 0.3	ND	0.93 ± 0.01
	Without simple purification	5.03	126.4 ± 8.3	115.3 ± 7.9	ND^b	ND	0.91 ± 0.01
	With simple purification	5.10	147.5 ± 1.5	138.4 ± 1.9	0.4 ± 0.1	ND	0.93 ± 0.01
Without simple purification	Without simple purification	4.98	106.8 ± 1.3^a	99.3 ± 0.6	3.2 ± 0.4	ND	0.93 ± 0.01
With simple purification	With simple purification	5.07	140.7 ± 7.5	129.4 ± 4.7	2.5 ± 0.2	ND	0.92 ± 0.01