Culture | B-1 | B-2 | B-3 |
---|---|---|---|
Aerobic stage | |||
 Carbon sources (g/L) | Glycerol 50a; ammonium acetate 10 | Glycerol 50b; ammonium acetate 10 | Glycerol 50c; ammonium acetate 10 |
 Time (h) | 38 | 36 | 28 |
 Aeration rate in induction stage (vvm) | 1.5 | 1.5 | 1.5 |
  μmax (batch)/(h) | 0.184 | 0.203 | 0.211 |
 Maximum cell concentration (g DCW/L) | 10.9 | 10.6 | 7.7 |
Anaerobic stage | |||
 Time (h) | 93 | 95 | 103 |
 Final concentration of succinate (mmol/L) | 50.3 | 566.0 | 615.9 |
 Final concentration of acetate (mmol/L) | 30.3 | 20.8 | 8.6 |
 Glycerol consumption (mmol/L) | 54.1 | 583.3 | 702.8 |
 Average specific succinate productivity (μmol g/DCW/h) | 49.6 | 562.0 | 773.2 |
 Average specific glycerol consumption rate (μmol g/DCW/h) | 53.3 | 579.3 | 882.4 |
 Yield of succinate in anaerobic stage (mol/mol) | 0.93 | 0.97 | 0.88 |