Fig. 2

Profiles of mAb charge variation distribution with different feed medium. a Acidic variant level; b basic variant level; c main variant level. (White bar) control condition; (shaded bar) uridine added condition; d CEX graph of antibodies in different fed-batch cultures. Red line represents mabs in control culture at day 14; blue line represents mab in uridine added culture at day 14. The error bars indicate the standard deviations from three independent experiments and p-values were estimated by two-tailed Student’s t test. *p < 0.05 relative to control; **p < 0.01 relative to control