Table 3 List of the structural and functional properties of pectin-deacetylating esterases belonging to CE-8 and CE-12 of carbohydrate esterases
Strain, PDB ID, CE class | Catalytic residues and reaction type | CATH and Pfam | Enzyme and fold Substrates and Refs |
|---|---|---|---|
Erwinia chrysanthemi, [1QJV], CE-8 | Asp178, Asp199, Arg267 Aspartic esterase | 3-Solenoid Pectinase lyase C-like pectinesterase | Pectin methyl esterase, single stranded right-handed β-helix, pectin present in cell wall polysaccharides is hydrolyzed to pectate and methanol (Jenkins et al. 2001) |
Aspergillus aculeatus, [1DEX], CE-12 | Ser9, His195, Asp192 Serine esterases | 3-Layer α/β sandwich Rossmann fold GDSL-like lipase/acylhydrolase | Rhamnogalacturonan acetylesterase flavodoxin-like (SGNH hydrolase). Rhamnogalacturonan present in the cell walls. RGAE functions in coordination with rhamnogalacturonan lyases and hydrolases (Mølgaard et al. 2000) |