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Table 3 Matrix of central composite design of independent variables for the production of biosurfactants
| Runs | Factors | Responses | ||||
|---|---|---|---|---|---|---|
| Peptone (g/L) | Temperature (°C) | Molasses (%, m/v) | Yield (g/L) | Inhibition diameter (mm) | Surface tension (mN/m) | |
| 1 | 8.00 (+ 1) | 40.00 (+ 1) | 9.00 (+ 1) | 1.00 | 21.50 | 49.91 |
| 2 | 5.50 (0) | 32.50 (0) | 5.50 (0) | 2.62 | 63.70 | 36.71 |
| 3 | 1.68 (− α) | 32.50 (0) | 5.50 (0) | 1.20 | 38.20 | 48.01 |
| 4 | 5.50 (0) | 32.50 (0) | 5.50 (0) | 2.60 | 62.78 | 36.98 |
| 5 | 5.50 (0) | 44.00 (+ α) | 5.50 (0) | 0.71 | 19.00 | 52.66 |
| 6 | 5.50 (0) | 32.50 (0) | 5.50 (0) | 2.74 | 63.60 | 36.01 |
| 7 | 3.00 (− 1) | 25.00 (− 1) | 9.00 (+ 1) | 1.00 | 22.82 | 49.09 |
| 8 | 5.50 (0) | 32.50 (0) | 0.16 (− α) | 1.20 | 33.07 | 48.72 |
| 9 | 9.31 (+ α) | 32.50 (0) | 5.50 (0) | 1.70 | 52.12 | 43.19 |
| 10 | 5.50 (0) | 32.50 (0) | 5.50 (0) | 2.36 | 54.12 | 39.13 |
| 11 | 8.00 (+ 1) | 40.00 (+ 1) | 2.00 (− 1) | 1.96 | 37.23 | 42.38 |
| 12 | 8.00 (+ 1) | 25.00 (− 1) | 9.00 (+ 1) | 0.90 | 23.12 | 51.28 |
| 13 | 3.00 (− 1) | 25.00 (− 1) | 2.00 (− 1) | 0.099 | 0.10 | 63.80 |
| 14 | 8.00 (+ 1) | 25.00 (− 1) | 2.00 (− 1) | 1.00 | 27.02 | 49.10 |
| 15 | 5.50 (0) | 32.50 (0) | 5.50 (0) | 2.35 | 64.90 | 38.07 |
| 16 | 5.50 (0) | 23.00 (− α) | 5.50 (0) | 0.30 | 5.80 | 54.80 |
| 17 | 5.50 (0) | 32.50 (0) | 5.50 (0) | 2.653 | 53.78 | 38.34 |
| 18 | 3.00 (− 1) | 40.00 (+ 1) | 9.00 (+ 1) | 1.30 | 32.70 | 46.78 |
| 19 | 5.50 (0) | 32.50 (0) | 10.83 (+ α) | 2.33 | 41.01 | 39.71 |
| 20 | 3.00 (− 1) | 40.00 (+ 1) | 2.00 (− 1) | 0.10 | 0.10 | 63.90 |
