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Table 3 Matrix of central composite design of independent variables for the production of biosurfactants

From: Application of response surface methodology to improve the production of antimicrobial biosurfactants by Lactobacillus paracasei subsp. tolerans N2 using sugar cane molasses as substrate

Runs Factors Responses
Peptone (g/L) Temperature (°C) Molasses (%, m/v) Yield (g/L) Inhibition diameter (mm) Surface tension (mN/m)
1 8.00 (+ 1) 40.00 (+ 1) 9.00 (+ 1) 1.00 21.50 49.91
2 5.50 (0) 32.50 (0) 5.50 (0) 2.62 63.70 36.71
3 1.68 (− α) 32.50 (0) 5.50 (0) 1.20 38.20 48.01
4 5.50 (0) 32.50 (0) 5.50 (0) 2.60 62.78 36.98
5 5.50 (0) 44.00 (+ α) 5.50 (0) 0.71 19.00 52.66
6 5.50 (0) 32.50 (0) 5.50 (0) 2.74 63.60 36.01
7 3.00 (− 1) 25.00 (− 1) 9.00 (+ 1) 1.00 22.82 49.09
8 5.50 (0) 32.50 (0) 0.16 (− α) 1.20 33.07 48.72
9 9.31 (+ α) 32.50 (0) 5.50 (0) 1.70 52.12 43.19
10 5.50 (0) 32.50 (0) 5.50 (0) 2.36 54.12 39.13
11 8.00 (+ 1) 40.00 (+ 1) 2.00 (− 1) 1.96 37.23 42.38
12 8.00 (+ 1) 25.00 (− 1) 9.00 (+ 1) 0.90 23.12 51.28
13 3.00 (− 1) 25.00 (− 1) 2.00 (− 1) 0.099 0.10 63.80
14 8.00 (+ 1) 25.00 (− 1) 2.00 (− 1) 1.00 27.02 49.10
15 5.50 (0) 32.50 (0) 5.50 (0) 2.35 64.90 38.07
16 5.50 (0) 23.00 (− α) 5.50 (0) 0.30 5.80 54.80
17 5.50 (0) 32.50 (0) 5.50 (0) 2.653 53.78 38.34
18 3.00 (− 1) 40.00 (+ 1) 9.00 (+ 1) 1.30 32.70 46.78
19 5.50 (0) 32.50 (0) 10.83 (+ α) 2.33 41.01 39.71
20 3.00 (− 1) 40.00 (+ 1) 2.00 (− 1) 0.10 0.10 63.90
  1. *() indicates the coded values of the different factors