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Table 1 Chemical extraction methods for recovery of chitin

From: Seafood waste: a source for preparation of commercially employable chitin/chitosan materials

S. no Method Type of conditions Properties of recovered chitin References
1. Chemical + enzymatic DM: 1.5 M HCl, ratio 1:10, at 50 °C for 6 h, DP: A21 protease enzyme/substrate 7, 75 U/mg (60 °C, 6 h) 88% deproteinated Younes et al. (2012)
2. Chemical + enzymatic DM: 0.55 M HCl, ratio of 1:10 (w/v) at room temperature
DP: B. mojavensis A21 crude protease (3 h, 50 °C, pH 9.0)
Degree of acetylation was 88.5% (shrimp) and 78.6% (crab shell) Hajji et al. (2014)
3. Chemical DM: 1 M HCl, ratio 1:30, 75 min at room temperature
DP: 3 M NaOH, ratio 1:30, 75 min at room temperature
  Srinivasan et al. (2018)
4. Chemical + enzymatic DM: 1.5 M of HCl (50 °C, 2 h)
DP: Alcalase (50 °C, 3 h)
80% deproteinated Abdelmalek et al. (2017)
5. Chemical DM: 1.0 M HCl (35 °C, 30 min)
DP: 2.0 M NaOH (80 °C, 20 h)
82% degree of acetylation Yong et al. (2018)
  1. DM demineralization, DP deproteination