Table 1 Chemical extraction methods for recovery of chitin
From: Seafood waste: a source for preparation of commercially employable chitin/chitosan materials
S. no | Method | Type of conditions | Properties of recovered chitin | References |
|---|---|---|---|---|
1. | Chemical + enzymatic | DM: 1.5 M HCl, ratio 1:10, at 50 °C for 6 h, DP: A21 protease enzyme/substrate 7, 75 U/mg (60 °C, 6 h) | 88% deproteinated | Younes et al. (2012) |
2. | Chemical + enzymatic | DM: 0.55 M HCl, ratio of 1:10 (w/v) at room temperature DP: B. mojavensis A21 crude protease (3 h, 50 °C, pH 9.0) | Degree of acetylation was 88.5% (shrimp) and 78.6% (crab shell) | Hajji et al. (2014) |
3. | Chemical | DM: 1Â M HCl, ratio 1:30, 75Â min at room temperature DP: 3Â M NaOH, ratio 1:30, 75Â min at room temperature | Â | Srinivasan et al. (2018) |
4. | Chemical + enzymatic | DM: 1.5 M of HCl (50 °C, 2 h) DP: Alcalase (50 °C, 3 h) | 80% deproteinated | Abdelmalek et al. (2017) |
5. | Chemical | DM: 1.0 M HCl (35 °C, 30 min) DP: 2.0 M NaOH (80 °C, 20 h) | 82% degree of acetylation | Yong et al. (2018) |