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Bioresources and Bioprocessing

Table 3 Total turnover numbers (TTNs) of mutants in the reduction of ketone 1

From: Laboratory evolution of an alcohol dehydrogenase towards enantioselective reduction of difficult-to-reduce ketones

Enzymes

TTNa

ee (%)b

WT

–

–

A85G

333

96(S)

I86A

482

80(S)

A85G/I86A

3071

> 99(S)

A85G/I86A/Q101A

6555

> 99(S)

A85G/I86A/C295A

5828

> 99(S)

  1. aTTN: [product formation]/[enzyme]
  2. bThe ee values were determined by chiral HPLC analysis; the absolute configuration was determined by comparison of the elution order with literature data (see Additional file 1)
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