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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Characterization of a recombinant thermotolerant argonaute protein as an endonuclease by broad guide utilization

Fig. 1

Phylogenetic tree and sequence analysis. a Neighbor-joining phylogenetic tree analysis of MfAgo based on amino acid sequences. The numbers at the nodes indicate the bootstrap values for neighbor-joining analysis of 1000 resampled data sets. The scale bar represents the evolutionary distance between species. Numbers in parentheses represent the sequence accession numbers in the NCBI database. b Multiple sequence alignments of MfAgo with other known pAgos. MjAgo: Ago from Methanocaldococcus jannaschii. PfAgo: Ago from Pyrococcus furiosus. AaAgo: Ago from Aquifex aeolicus. TtAgo: Ago from Thermus thermophilus. Only regions containing the DEDX catalytic residues (indicated in red) are shown. The alignment was carried out using ClustalW, shaded manually and using ESPript 3.0 (Robert and Gouet 2014)

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