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Table 3 Residues of MfAgo to interact with the 5ʹ end of the guide

From: Characterization of a recombinant thermotolerant argonaute protein as an endonuclease by broad guide utilization

Guide

MfAgo

Distance (Å)

Type

Atom

Residue

Atom

5′-OH ssDNA

O5′

Asp433

Oδ1

2.8

5′-P ssDNA

OP2

Arg428

Nη1

2.9

OP2

Asp433

Oδ1

2.5

O4

Lys685

Nζ

3.3

5′-P ssRNA

OP2

Arg428

Nη1

2.9

OP2

Asp433

Oδ1

2.5

O4

Lys685

Nζ

3.3