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Bioresources and Bioprocessing

Table 2 A list of OsmY-DyP4 variants and the mutations in DyP4-coding sequences verified by DNA sequencing

From: Accelerated directed evolution of dye-decolorizing peroxidase using a bacterial extracellular protein secretion system (BENNY)

OsmY-DyP4 and variants

Mutagenic rate of epPCR library

Missense mutations

Silent mutations

OsmY-WT*

N/A

N/A

N/A

OsmY-1D2*

L

N312S (A→G)

D241 (T→C)

I444 (C→T)

OsmY-1D7

H

A306V (C→T)

R323 (T→C)

OsmY-2A5*

M

I56V (A→G)

N312S (A→G)

D241 (T→C)

I444 (C→T)

G73 (T→A)

L245 (G→T)

OsmY-2C2

M

H374R (A→G)

N312S (A→G)

D241 (T→C)

I444 (C→T)

OsmY-2F8

H

N227D (A→G)

N312S (A→G)

D241 (T→C)

I444 (C→T)

OsmY-3F6*

L

K109R (A→G)

N312S (A→G)

I56V (A→G)

D241 (T→C)

I444 (C→T)

G73 (T→A)

L245 (G→T)

OsmY-4D4

N/A

N227S

N312S (A→G)

I56V (A→G)

K109R (A→G)

D241 (T→C)

I444 (C→T)

G73 (T→A)

L245 (G→T)

  1. Clones labelled with an asterisk * were used as parental template for the subsequent round of mutagenesis; mutations in italics represent nucleotide substitutions added to a parental template
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