Fig. 5
Effects of temperature on the specific activity (a) and thermostability (b) of AfPase. The reactions were performed in 100 mM HEPES buffer (pH 7.0) containing 1 mM p-nitrophenyl phosphate, 5 mM MgCl2, and appropriate concentration of phosphatase at different temperatures (a). The enzyme (1 g/L) was preincubated at different temperatures in 50 mM HEPES buffer (pH 7.0). The residual activity was measured at 70 °C using p-nitrophenyl phosphate as substrate. Thermal inactivation is plotted on logarithmic scale to demonstrate first-order kinetics (b)