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Parthenolide production in cell suspension culture of feverfew

Abstract

Feverfew (Tanacetum parthenium) is one of the most important medicinal plants with different pharmacologic properties, such as anti-inflammatory, cardiotonic, antitumor and antiangiogenic activities. Parthenolide (PN) is a main bioactive molecule in feverfew which belongs to sesquiterpene lactone compounds. Currently, the plant cell suspension has been used as a useful method to produce secondary metabolites (SMs) components. Meanwhile, the elicitor application is an effective strategy to induce the production of SMs in plants. The present study was conducted as two different experiments in cell suspension of feverfew. In the first experiment, the effects of explant (shoot and root), hormone (TDZ + NAA and TDZ + 2. 4-D) on cell dry weight for one month were investigated. In the second experiment, the effect of elicitor (namely, MJ, YE and Ag+) and the hormones after 24, 48 and 72 h on PN content was assessed. The result of the first experiment revealed that the simple effects and the interaction of hormone × explant were significant (P < 0.01) for cell dry weight. Growth rate analysis showed that shoot-derived cell suspension in 1 mg L−1 NAA + 0.5 mg L−1 TDZ treatment had the highest amount of cell dry weight 14 days after the culture. According to the second experiment, the highest PN content was obtained in cell suspension containing 0.5 mg L−1 2, 4-D + 0.1 mg L−1 TDZ with application of the YE + MJ elicitor after 48 h. The cell suspension treatment with each of the elicitors had a positive effect on the PN production. In conclusion, the application of combined elicitors in feverfew cell suspension culture can be used as an efficient tool for large-scale PN production.

Introduction

Feverfew (Tanacetum parthenium) is one of the most important medicinal plants with different pharmacological and therapeutic properties (Pourianezhad et al. 2016). It is a perennial plant of Asteraceae family. The plant is native to Kazakhstan, Central Asia and Mediterranean region, but it can be found in different parts of Europe, Asia and America (Arabasi and Bayram 2005; Farzaneh et al. 2002). The plant has been used to treat a wide range of diseases such as menstrual disorders, toothache, migraine, asthma, fever, stomach ache, arthritis, insect bites and helminthiasis (Maggi 2019; Sadat-Hosseini et al. 2017). Secondary metabolites (SMs) have complex structures to be manufactured by chemical synthesis and thus frequently extracted from naturally grown or cultivated plants (Deepthi and Satheeshkumar 2016). SMs are produced by different medicinal plants under biotic and abiotic stresses (Ebrahimi et al. 2012; Farajpour et al. 2017). Parthenolide (PN) is one of the major bioactive components of Feverfew. It is well known to have potential anti-inflammatory properties (Zhang et al. 2004). PN, a sesquiterpene lactone, is mostly produced by the mevalonate (MVA) pathway (Fig. 1). In this pathway, acetyl-CoA is converted to isopentenyl pyrophosphate (IPP) in six steps. Then, two IPPs and one dimethylallyl pyrophosphate (DMAPP) are transformed to farnesyl diphosphate (FPP), which is precursor for all sesquiterpenes, through farnesyl diphosphate synthase (FPS). Afterward, sesquiterpene synthase converted FPP to sesquiterpenes, which are further modified by oxidation reactions using cytochrome P450 enzymes. PN has a germacranolide backbone structure. Germacrene A oxidation to germacranoic acid in feverfew is usually accomplished by a similar P450 monooxygenase (Majdi et al. 2011; van Klink et al. 2003). Transcription level of SMs-related genes determines the biosynthesis and accumulation of SMs in plants, which can occur in different growth phases and places such as organs, tissues (De Kraker et al. 2002). Different parts of feverfew had different amounts of PN, so that the maximum PN content was detected in flower head and leaves (1.38 and 0.95%, respectively), while the PN contents in stalks and roots were 0.08 and 0.01%, respectively (Heptinstall et al. 1992). Currently, the plant cell culture has been used as a useful method for SMs components production (Zhong 2001). It is a promising alternative approach instead of traditional methods (Yue et al. 2016). High SM production is important for the commercial utilization of cell suspension cultures. There are many reports about increasing the SM production in cell cultures via some culture parameters such as elicitation, hormone, medium culture and precursor feeding (Khan et al. 2018).

Fig. 1
figure1

Presumed biosynthetic pathway of parthenolide; step 1 is catalyzed by germacrene A synthase; steps 2, 3 and 4 are catalyzed by germacrene A oxidase; step 5a, 5b, costunolide synthase; and step 6, costunolide epoxidase/parthenolide synthase (Majdi et al. 2011)

The mechanism of induction of SMs production by elicitors is same as the biotic/abiotic stresses. Elicitors activate a range of defense mechanisms, including the production and accumulation of an array of plant-defensive SMs in plants or cell suspension cultures (Zhao et al. 2005). There are several different components that can completely substitute for biotic or abiotic elicitors in the elicitation effect. Moreover, several binding sites for protein or peptides elicitors were also characterized in different plants (Zhai et al. 2017). Elicitors were used in many studies as an enhanced biomass production in different plants in vitro culture such as Ophiorrhiza mungos (Deepthi and Satheeshkumar 2016), Silybum marianum (Gabr et al. 2016), Glycyrrhiza uralensis (Wang et al. 2017), Eruca sativa (Kastell et al. 2018), Isatis tinctoria (Gai et al. 2019) and Centella asiatica (Gupta and Chaturvedi 2019). There are many elicitors such as N,N′-dicyclohexylcarbodiimide and the derivatives of methyl jasmonate, salicylate and 2,6-dichloroisonicotinate as novel and powerful inducers for plant secondary metabolism (Huang et al. 2013; Qian et al. 2006a, b). However, among them, methyl jasmonate and salicylate elicitors were most used in different medicinal plants (Gai et al. 2019; Hao et al. 2015; Krzyzanowska et al. 2012; Lee-Parsons et al. 2004; Majdi et al. 2015). Elicitors such as MJ, SA, yeast extract (YE) and Ag+ could be an effective tool to increase PN production because they are not destructive for plant tissues related to terpene accumulation (Majdi et al. 2011). Leaves of T. parthenium were elicited using methyl jasmonate (MJ) and salicylic acid (SA), and both elicitors activated PN biosynthesis (Majdi et al. 2015). We have previously investigated the effects of various elicitors including yeast extract (YE) (2.5 mg L−1), methyl jasmonate (MJ) (100 µM), Ag+ (100 µM), YE + MJ, Ag+ + MJ, YE + Ag+ and YE + MJ + Ag+ on the production of PN in feverfew hairy root culture (Pourianezhad et al. 2019). We obtained the highest values of PN contents by application of different combined elicitors. The aims of the current study were to (1) determine the appropriate time for applying the elicitors in suspension culture and (2) investigate the effect of elicitors and their combinations, namely MJ, YE, Ag+, MJ + YE, MJ + Ag+, YE + Ag+ and MJ + YE + Ag+, on PN content in T. parthenium suspension culture.

Materials and methods

Plant material and callus induction

Seeds were obtained from Isfahan Pakan Bazr Company and surface-sterilized by soaking in 70% ethanol for 30 s and immersing in 2% sodium hypochlorite for 5 min. Afterward, the seeds were washed three times with sterile distilled water (SDW) and germinated on ¼ strength MS medium containing 3 and 0.8% (w/v) sucrose and agar, respectively, at pH 5.7 (Murashige and Skoog 1962). The cultures were kept in an incubator at 25 °C with 16:8-h long-day (LD) photoperiod and white fluorescent tubes (ca. 40 µmol m−2 s−1) on ½ strength MS medium. In order to induce the callus formation, the sterilized explants (leaf and stem) were cut into 5-mm sections and placed on ½ strength MS solid medium containing different concentrations of TDZ (0, 0.1 and 0.5 mg L−1), NAA (0, 0.5, 1 and 2 mg L−1) and 2,4-D (0, 0.5, 1 and 2 mg L−1) supplemented with 30 g L−1 sucrose. After four weeks, the induced callus was detached from explants and separately cultured until being used in cell suspension culture.

Cell suspension culture and growth curve analysis

According to the results of callus induction experiments (data not shown), the two best hormonal treatments including 1 mg L−1 NAA and 0.5 mg L−1 TDZ, and 0.5 mg L−1 2,4-D and 1 mg L−1 TDZ were selected for cell suspension culture. To this aim, about 2 g of selected fresh callus was transferred to a 500-mL Erlenmeyer flask containing 100 mL of culture medium and incubated at room temperature (25 °C) on a rotary shaker (110 rpm) in darkness. Subculture was performed at 2-week interval by adding 30 mL of each suspension culture to an Erlenmeyer flask containing 70 mL of fresh culture medium.

The growth of the cells in suspension cultures was studied by measurement of cell dry weight for 30 days. Five milliliters of suspension cultures was added to 100-mL Erlenmeyer flask containing 20 mL of ½ strength MS liquid medium supplemented with the same hormonal concentrations, and the cultures were incubated on a rotary shaker at 90 rpm. At every two days, 1.5 mL of suspension cultures was filtered through Whatman filter paper and dried at 60 °C for 24 h and cell dry weights were measured.

Elicitors preparation and application

In the present study, three elicitors and their combinations were used. The elicitors included yeast extract (YE, 2.5 mg L−1), methyl jasmonate (MJ, 0.5 mg L−1), AgNO3 (Ag+, 0.5 mg L−1), YE + MJ, Ag++MJ, YE + Ag+ and YE + MJ + Ag+. The elicitors were added to the suspension cultures at day 14. After 24, 48 and 72 h of elicitor application, the cell biomass was frozen and lyophilized for PN content measurement. YE and AgNO3 were dissolved in SDW and methyl jasmonate in 70% (v/v) ethanol. Membrane filter (0.2 µm) was used to sterilize the solutions and then they were stored at − 20 °C.

PN measurement

Lyophilized cell biomass was powdered in liquid nitrogen using a chilled mortar and pestle. The PN content was measured according to a protocol described by Jeong et al. (Jeong et al. 2005). All details about the used method can be found in our previous report (Pourianezhad et al. 2019).

Statistical analysis

In the present study, two different experiments were carried out. The first experiment was a completely randomized factorial design to study the effects of hormone, explant and time on cell dry weight. In the second one, the effects of hormone, elicitor and time on PN content were evaluated. The ANOVA and LSD tests were applied for statistical assessments using Statistical Analysis System (SAS, v9.1 for Windows, SAS Institute Cary, NC).

Results and discussion

Effects of hormone and explant on cell dry weight

According to our previous study, different hormonal treatments were used for callus induction in feverfew. Based on the results (data not shown), two hormonal treatments including 1 mg L−1 NAA + 0.5 mg L−1 TDZ and 0.5 mg L−1 2,4-D + 0.1 mg L−1 TDZ were selected for cell suspension culture. The hormonal treatments were applied to shoot- and root-derived cell suspension cultures, and every two days, cell dry weight was measured for one month. ANOVA revealed that the simple effects and the interaction of hormone × explant were significant (P < 0.01) for cell dry weight in feverfew cell suspension culture, while other interactions were nonsignificant (Table 1). Based on the mean comparison results, the highest amount of cell dry weight was obtained from shoot-derived cell suspension in 1 mg L−1 NAA + 0.5 mg L−1 TDZ treatment, which was significantly different from the other treatments. No significant differences were observed among the other treatments (Fig. 2). Modarres et al. (2018) established a cell suspension culture of Salvia leriifolia to induce the bio-production of phenolic acids. They obtained the highest rate of callus induction (100%) and callus dry weight (0.38 g) in medium containing 5 mg L−1 NAA and 6-benzylaminopurine. Studies have revealed that the plant growth and secondary metabolite production were affected by the auxin and cytokinin types and their concentrations (Rao and Ravishankar 2002). Time-course analysis of cell growth rate revealed a lag period of 10 days in shoot-derived cell suspension in TDZ + NAA treatment. A logarithmic growth from days 4–14 was detected and then declined. The other treatments showed no logarithmic phase, and therefore, the best time for applying the elicitors was selected based on this treatment. Furthermore, growth curve analysis showed that shoot-derived cell suspension in TDZ + NAA treatment had the highest amount of cell DW at day 14 (Fig. 3).

Table 1 Analysis of variance of the effect of hormone, explant/elicitor and time on two measured characters of feverfew cell suspension culture
Fig. 2
figure2

Effect of hormone and explant on cell dry weight (g) of feverfew cell suspension culture. Means with different letters were significantly different according to the LSD test

Fig. 3
figure3

Time-course analysis of growth rate in cell suspension cultures of T. parthenium in 30-day period

Effects of elicitors on PN production

The elicitors were applied to cell suspension 14 days after culture. After 24, 48 and 72 h of elicitor application, their effects on PN content were investigated. ANOVA showed that the main effects and their interactions were significant (P < 0.01) (Table 1). According to Wickens and Keppel (2004), when the interaction of three factors was significant, less attention is paid to the main effects and we should focus on the interaction effect. Therefore, in the present study, we have focused on the interaction of the three studied factors. The maximum PN production (0.066 mg g−1 DW) was observed in 0.5 mg L−1 2,4-D + 0.1 mg L−1 TDZ treated by YE + MJ after 48 h, followed by the same treatment after 24 h (0.060 mg g−1 DW). Meanwhile, the minimum PN production was observed in control treatment (Fig. 4). According to Fig. 4, all applied elicitors significantly increased the PN production compared to the control treatment. In addition, the result showed that the highest PN content was achieved 48 h after elicitor application compared to the other times. PN is one of the most imperative sesquiterpene lactones of bioactive compounds in feverfew. The PN biosynthesis regulation process by elicitors is still unknown, while its production is highly genotype dependent (Stojakowska and Kisiel 1997). Methyl jasmonate and salicylic acid stimulate the biosynthesis of many secondary metabolites such as sesquiterpenes, phenols and indole alkaloids (Matkowski 2008; Yu et al. 2006). YE, AgNO3 and MJ elicitors influenced dry weight and secondary metabolites production in various plant species such as Ophiorrhiza mungos, Mentha piperita and Hypericum perforatum (Deepthi and Satheeshkumar 2016; Krzyzanowska et al. 2012; Wang et al. 2015). According to the results, combined elicitors were more useful for PN production. In our previous study, the effects of YE, MJ, Ag elicitors and their combinations on PN production in feverfew hairy root culture were explored (Pourianezhad et al. 2019). The results of the study showed that the highest PN content was achieved after application of YE and MJ combined elicitors. In the present and our previous studies, the best treatment for PN production was YE and MJ combined elicitors; however, the PN content in the cell suspension culture was more than hairy root culture. The reason behind the more positive effect of combined elicitors on PN production compared to the separate elicitor application is not yet clear. Whatever it is, the application of combined elicitors can be more useful to the production of parthenolide than separately applied elicitors, which may be due to the interactions between different physiological processes (Zhao et al. 2001).

Fig. 4
figure4

Effect of hormonal combination, elicitors on parthenolide production in feverfew cell suspension culture after 24, 48 and 72 h of elicitor application. Means with different letters were significantly different according to the LSD test

Conclusion

We successfully established a cell suspension culture in feverfew for the PN production. The highest amount of cell dry weight was obtained from shoot-derived cell suspension in 1 mg L−1 NAA + 0.5 mg L−1 TDZ treatment. Furthermore, the result showed that the highest PN content was obtained in cell suspension containing 0.5 mg L−1 2,4-D + 0.1 mg L−1 TDZ with application of the YE + MJ elicitor after 48 h. The cell suspension treatment with each of the elicitors had a positive effect on the PN production. In conclusion, the application of combined elicitors in cell suspension culture of feverfew can be used as an efficient tool to increase the PN production.

Availability of data and materials

All data generated or analyzed during this study are included in this article.

Abbreviations

PN:

parthenolide

YE:

yeast extract

MJ:

methyl jasmonate

SM:

secondary metabolites

HPLC:

high-performance liquid chromatography

SA:

salicylic acid

LSD:

least significant difference

ANOVA:

analysis of variances

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HR and AM conceived and designed the research. FP conducted experiments and wrote the manuscript. MK and SM were involved in results elaboration and discussion, and critical reading of the manuscript. All authors read and approved the final manuscript.

Correspondence to Amir Mousavi.

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Keywords

  • Elicitors
  • Feverfew (Tanacetum parthenium)
  • Cell suspension culture
  • Parthenolide