Fig. 1
From: Reversible photocontrol of oxidase activity by inserting a photosensitive domain into the oxidase
Designing alovX (X present different insertion sites) photoswitches. a Crystal structure analysis of wild-type AdhP protein to select surface-exposed loop (red represents active center of AdhP. Magenta represents exposed sits in surface loop). b Screening unconservative sites from surface-exposed loops by sequence alignment (yellow star represents the selected site. The information of ten sites at Additional file 1: Figure S3). c Comparing catalytic activity between WT AdhP and alov1-10 fusions in the dark state. Error bars show SEM (n = 3)