Fig. 8
From: Cell-culture growth conditions resulting in the oxidation of a recombinant antigen-binding fragment
a ESI–MS spectrum of rFab from 5 L bioreactor under non-reduced condition. The MW profile of rFab was observed at 48,813 Da, and no oxidized rFab was detected from 5 L bioreactor. b ESI–MS spectrum of rFab from 5 L bioreactor under TCEP-reduced condition. The MW at 23,587 Da matched with the light-chain theoretical MW with two disulfide bonds, and the MW at 25,229 Da matched with the heavy chain theoretical MW with two disulfide bonds