Table 1 Different eluent compositions

Chloroform/methanol/glacial acetic acid/water = 26/10/0.8/0.7 (v/v/v/v)

N/A^a

Chloroform/methanol/glacial acetic acid/water = 26/10/0.8/1.1 (v/v/v/v)

N/A^a

Chloroform/methanol/glacial acetic acid/water = 26/10/0.8/1.5 (v/v/v/v)

23.52 ± 0.02

94.71 ± 3.64

Chloroform/methanol/glacial acetic acid/water = 26/10/0.8/3 (v/v/v/v)

N/A^b