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Table 1 Different eluent compositions

From: Preparation and purification of novel phosphatidyl prodrug and performance modulation of phosphatidyl nanoprodrug

Eluent compositions Recovery (%) Purity (%)
Chloroform/methanol/glacial acetic acid/water = 26/10/0.8/0.7 (v/v/v/v) N/Aa
Chloroform/methanol/glacial acetic acid/water = 26/10/0.8/1.1 (v/v/v/v) N/Aa
Chloroform/methanol/glacial acetic acid/water = 26/10/0.8/1.5 (v/v/v/v) 23.52 ± 0.02 94.71 ± 3.64
Chloroform/methanol/glacial acetic acid/water = 26/10/0.8/3 (v/v/v/v) N/Ab
  1. aN/A, not available. Owing to the low polarity of the eluent, PMA could not be eluted
  2. bN/A, not available. Because separation of PMA from the impurities and PC was not achieved