Table 2 Different gradient eluent conditions (quantitative analysis by HPLC, flow rate = 3.5 mL/min)
Gradient eluent system | Time (min) | The composition ratio of eluent (Chloroform/methanol/glacial acetic acid/water) (v/v/v/v) | Recovery (%) | Purity (%) |
|---|---|---|---|---|
1 | 0 | 26/10/0.8/0.7 | 36.75 ± 0.03 | 96.06 ± 2.18 |
100 | 26/10/0.8/2 | |||
160 | End | |||
2 | 0 | 26/10/0.8/0.7 | 38.62 ± 0.02 | 93.97 ± 2.13 |
100 | 26/10/0.8/2 | |||
120 | 26/10/0.8/3 | |||
160 | End | |||
3 | 0 | 26/10/0.8/0.7 | 50.35 ± 0.02 | 96.93 ± 1.9 |
100 | 26/10/0.8/3 | |||
150 | End | |||
4 | 0 | 26/10/0.8/1.1 | 68.25 ± 0.03 | 69.02 ± 2.25 |
20 | 26/10/0.8/3 | |||
70 | End |