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Table 2 Different gradient eluent conditions (quantitative analysis by HPLC, flow rate = 3.5 mL/min)

From: Preparation and purification of novel phosphatidyl prodrug and performance modulation of phosphatidyl nanoprodrug

Gradient eluent system Time (min) The composition ratio of eluent (Chloroform/methanol/glacial acetic acid/water) (v/v/v/v) Recovery (%) Purity (%)
1 0 26/10/0.8/0.7 36.75 ± 0.03 96.06 ± 2.18
100 26/10/0.8/2
160 End
2 0 26/10/0.8/0.7 38.62 ± 0.02 93.97 ± 2.13
100 26/10/0.8/2
120 26/10/0.8/3
160 End
3 0 26/10/0.8/0.7 50.35 ± 0.02 96.93 ± 1.9
100 26/10/0.8/3
150 End
4 0 26/10/0.8/1.1 68.25 ± 0.03 69.02 ± 2.25
20 26/10/0.8/3
70 End