Fig. 2
Validation of C. necator H16 metabolic model. Specific rates and yields for cell growth simulated by flux balance analysis of the metabolic model under heterotrophic or autotrophic growth were compared against reported experimental data. Yields were obtained under different conditions of substrate uptake (A: qfructose = 0.08 g/g-cell-h; B: qfructose = 0.14 g/g-cell-h; C: qfructose = 0.47 g/g-cell-h; D: qco2 = 0.19 g/g-cell-h; E: qco2 = 0.02 g/g-cell-h). Heterotrophy was based on growth on fructose, while autotrophy was based on growth on CO2 as sole carbon source. Consistency between the experimental observation and the model prediction confirms the accuracy of the constructed metabolic model