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Table 4 Cell culture performance comparison between fed-batch Process C and Process A or B for different mAb production on Day 14 unless otherwise specified

From: Development of an intensified fed-batch production platform with doubled titers using N-1 perfusion seed for cell culture manufacturing

ProductProcess at bioreactor scaleTiter (normalized weight/L)Peak VCD (× 106 cells/mL)Cell specific productivity (normalized weight/cell/day)Volumetric productivity (normalized weight/L/day)
mAb1B at 1000-L (n = 5)0.5 ± 0.0324.0 ± 2.72.05 ± 0.200.035 ± 0.002
C at 500-L (n = 2)1.00 ± 0.0229.7 ± 1.32.98 ± 0.060.071 ± 0.002
mAb2 on Day 10B at 1000-L (n = 5)0.50 ± 0.0219.5 ± 1.13.03 ± 0.210.042 ± 0.002
C at 5-L (n = 4)1.00 ± 0.0133.4 ± 1.63.04 ± 0.070.084 ± 0.001
mAb3B at 1000-L (n = 3)0.53 ± 0.0222.3 ± 0.32.24 ± 0.050.034 ± 0.000
C at 500-L (n = 2)1.00 ± 0.0540.9 ± 5.02.41 ± 0.360.071 ± 0.001
mAb4A at 1000-L (n = 5)0.31 ± 0.0332.9 ± 2.71.05 ± 0.140.022 ± 0.002
C at 5-L (n = 4)1.00 ± 0.0242.3 ± 1.32.28 ± 0.080.071 ± 0.002
C at 500-L on Day 12 (n = 1)1.00472.240.083
  1. The Process C final titer at 500-L scale was normalized as 1 for each of mAb1, mAb3 and mAb4, while the Process C titer at 5-L scale on Day 10 was normalized as 1 for mAb2