Table 1 The plasmids and primers used for reverse mutation
From: Engineering a diaphorase via directed evolution for enzymatic biofuel cell application
Description | Contents |
|---|---|
Plasmids | GsDI mutant 3–8 |
Primers | |
37-F | AACAAGTGCATCCGGACCATGAGGTGATCCATCTCGATT |
37-R | AATCGAGATGGATCACCTCATGGTCCGGATGCACTTGTT |
61-F | CGACGTGTTCAGCGGCTGGGGCAAACTTCGCTCCGGGAA |
61-R | TTCCCGGAGCGAAGTTTGCCCCAGCCGCTGAACACGTCG |
68-F | CAAACTTCGCTCCGGGAAATCGTTTGAAGAGCTGACTGT |
68-R | ACAGTCAGCTCTTCAAACGATTTCCCGGAGCGAAGTTTG |
73-F | GAAAACGTTTGAAGAGCTGTCTGTCGAAGAAAAAGCGAA |
73-R | TTCGCTTTTTCTTCGACAGACAGCTCTTCAAACGTTTTC |
74-F | ACGTTTGAAGAGCTGACTGACGAAGAAAAAGCGAAAGTC |
74-R | GACTTTCGCTTTTTCTTCGTCAGTCAGCTCTTCAAACGT |
105-F | CGTCACGCCGATGTGGAACTTTTCGTTCCCGCCGGTGTT |
105-R | AACACCGGCGGGAACGAAAAGTTCCACATCGGCGTGACG |