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Table 3 The activity of reverse amino acid residue

From: Engineering a diaphorase via directed evolution for enzymatic biofuel cell application

Site

Reverse amino acid residue

37

61

68

73

74

105

M 3–8

Sp.act (U mg−1)

0.59 ± 0.05

0.44 ± 0.05

0.50 ± 0.06

0.59 ± 0.09

0.47 ± 0.02

0.16 ± 0.02

0.61 ± 0.1

  1. Enzyme activity assayed at pH 4.5