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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: A method for high-throughput screening hydrolase of lignin β-aryl ether linkage from directed evolution by glutathione (GSH) assay

Fig. 5

Enzymatic activity of screened mutants analyzed by HPLC. B7, C1, C3, E1, F1, F6, H11, AA4, AE4, AF1, AH8, BE1, BG5 and BG11 are the mutants described in Table 2. DE3 refers to the cell lysate from BL21(DE3) with pET-23a plasmid. The green column refers to the original LigF. The blue columns refer to the positive mutants with increased activity selected by GSH assay, while the red columns refer to the negative mutants with decreased activity selected by GSH assay

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