Table 1 Strains and plasmids used in this study
Name | Description | Reference or source |
|---|---|---|
Strains | ||
DH5α | Plasmids storage strain | Taihe Biotechnology CO., Ltd. |
BL21(DE3) | Protein expression strain | Taihe Biotechnology CO., Ltd. |
Plasmids | ||
pET-23a | Protein expression vector | Novegan |
pET-23a-ligD | The expression vector of LigD protein | Wang et al. (2016) |
pET-23a-ligF | The expression vector of LigF protein | Wang et al. (2016) |