Table 1 Plasmids and strains used in this study
Plasmid | Description | Reference or source |
|---|---|---|
 pAG32 | AmpicillinR; HygromycinR | Liu et al. (2018b) |
 pPICZB | ZeocinR | Invitrogen |
 pAGA | pAG32 derivative containing PAOX1 | This study |
 pZ_PGAP-tapA | pGAPZ α derivative containing tapA | Liu et al. (2018a) |
 pG_TapA | pAGA derivative containing tapA | This study |
 pG_sMlcE | pAGA derivative containing mlcE (codon optimized for K. phaffii) | This study |
 pG_sLovI | pAGA derivative containing lovI (codon optimized for K. phaffii) | This study |
 pG_sMokI | pAGA derivative containing mokI (codon optimized for K. phaffii) | This study |
 pG_sTapA | pAGA derivative containing tapA (codon optimized for K. phaffii) | This study |
Strain | Genotype | Reference or source |
|---|---|---|
 J#9 | D#9 with plasmid p3.5 K-sAR | Liu et al. (2018a) |
 J#9-TapA | J#9 with plasmid pG_TapA | This study |
 J#9-sMlcE | J#9 with plasmid pG_sMlcE | This study |
 J#9-sLovI | J#9 with plasmid pG_sLovI | This study |
 J#9-sMokI | J#9 with plasmid pG_sMokI | This study |
 J#9-sTapA | J#9 with plasmid pG_sTapA | This study |
 GS115 | his4 | Invitrogen |
 GS-PGAP-TapA | GS115 with plasmid pGAPZα-TapA | Liu et al. (2018a) |